Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1995299..1996130 Replicon chromosome
Accession NZ_CP028770
Organism Escherichia coli strain A4

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag AOY55_RS09425 Protein ID WP_000854814.1
Coordinates 1995299..1995673 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag AOY55_RS09430 Protein ID WP_001285586.1
Coordinates 1995762..1996130 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY55_RS09385 (1990695) 1990695..1991861 + 1167 WP_001519880.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
AOY55_RS09390 (1991980) 1991980..1992453 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
AOY55_RS09395 (1992651) 1992651..1993709 + 1059 WP_001200890.1 FUSC family protein -
AOY55_RS09400 (1993881) 1993881..1994210 + 330 WP_000450409.1 DUF496 family protein -
AOY55_RS09405 (1994311) 1994311..1994445 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
AOY55_RS09410 (1994547) 1994547..1994693 + 147 Protein_1847 transposase domain-containing protein -
AOY55_RS09415 (1994982) 1994982..1995062 - 81 Protein_1848 hypothetical protein -
AOY55_RS09420 (1995108) 1995108..1995302 - 195 WP_000988601.1 DUF5983 family protein -
AOY55_RS09425 (1995299) 1995299..1995673 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
AOY55_RS09430 (1995762) 1995762..1996130 - 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
AOY55_RS09435 (1996204) 1996204..1996425 - 222 WP_000692323.1 DUF987 domain-containing protein -
AOY55_RS09440 (1996494) 1996494..1996970 - 477 WP_001351157.1 RadC family protein -
AOY55_RS09445 (1996986) 1996986..1997459 - 474 WP_000855074.1 antirestriction protein -
AOY55_RS09450 (1997722) 1997722..1998543 - 822 WP_001234571.1 DUF932 domain-containing protein -
AOY55_RS09455 (1998764) 1998764..1999174 - 411 WP_000846704.1 hypothetical protein -
AOY55_RS09460 (1999190) 1999190..1999867 - 678 WP_104214272.1 hypothetical protein -
AOY55_RS09465 (2000003) 2000003..2001073 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T104039 WP_000854814.1 NZ_CP028770:c1995673-1995299 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T104039 NZ_CP028770:c1995673-1995299 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT104039 WP_001285586.1 NZ_CP028770:c1996130-1995762 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT104039 NZ_CP028770:c1996130-1995762 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References