Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4332542..4333039 Replicon chromosome
Accession NZ_CP028769
Organism Escherichia coli strain A5

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY56_RS20825 Protein ID WP_001173343.1
Coordinates 4332542..4332715 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag AOY56_RS20830 Protein ID WP_001425728.1
Coordinates 4332743..4333039 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY56_RS20815 (4329212) 4329212..4330669 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
AOY56_RS20820 (4330906) 4330906..4332423 + 1518 WP_001295074.1 lysine--tRNA ligase -
AOY56_RS20825 (4332542) 4332542..4332715 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY56_RS20830 (4332743) 4332743..4333039 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY56_RS20835 (4333266) 4333266..4333538 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
AOY56_RS20840 (4333550) 4333550..4333780 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
AOY56_RS20845 (4333961) 4333961..4335592 + 1632 WP_001216487.1 sensor histidine kinase -
AOY56_RS20850 (4335589) 4335589..4336308 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T104032 WP_001173343.1 NZ_CP028769:c4332715-4332542 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T104032 NZ_CP028769:c4332715-4332542 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT104032 WP_001425728.1 NZ_CP028769:c4333039-4332743 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT104032 NZ_CP028769:c4333039-4332743 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References