Detailed information of TA system TA104030

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4169319..4170026
• Replicon: chromosome
• Accession: NZ_CP028769
• Organism: Escherichia coli strain A5

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: AOY56_RS20030
• Protein ID: WP_086629380.1
• Coordinates: 4169319..4169660 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AOY56_RS20035
• Protein ID: WP_086629383.1
• Coordinates: 4169691..4170026 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY56_RS20010 (4165308)	4165308..4166573	-	1266	WP_001218329.1	integrase arm-type DNA-binding domain-containing protein	-
AOY56_RS20015 (4166966)	4166966..4167913	-	948	WP_032251793.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
AOY56_RS20020 (4167906)	4167906..4168301	-	396	WP_000152739.1	DUF6088 family protein	-
AOY56_RS20025 (4168371)	4168371..4169204	-	834	WP_086629378.1	DUF4942 domain-containing protein	-
AOY56_RS20030 (4169319)	4169319..4169660	-	342	WP_086629380.1	TA system toxin CbtA family protein	Toxin
AOY56_RS20035 (4169691)	4169691..4170026	-	336	WP_086629383.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AOY56_RS20040 (4170026)	4170026..4170499	-	474	WP_086629385.1	DNA repair protein RadC	-
AOY56_RS20045 (4170529)	4170529..4171347	-	819	WP_001296926.1	DUF932 domain-containing protein	-
AOY56_RS20050 (4171584)	4171584..4172537	-	954	WP_087599172.1	hypothetical protein	-
AOY56_RS20055 (4173130)	4173130..4173744	+	615	WP_087599171.1	inovirus Gp2 family protein	-
AOY56_RS20060 (4173862)	4173862..4174080	+	219	WP_001064742.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimC / fimI / fimA / fimE / fimB	4136680..4186231	49551

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13048.07 Da
• Isoelectric Point: 9.5454

>T104030 WP_086629380.1 NZ_CP028769:c4169660-4169319 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAINFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRCHNTTAR

Nucleotide

• Length: 342 bp

>T104030 NZ_CP028769:c4169660-4169319 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ATGCCGGAATCACTCTGGTCGATGCCATCAATTTTTTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTACGGGCGCGGCAGGCTACAGGTCTGCTGCGCCGCTG
TCATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12613.65 Da
• Isoelectric Point: 8.1281

>AT104030 WP_086629383.1 NZ_CP028769:c4170026-4169691 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADDELSPCQQR
TVTLYAKGLTCKADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT104030 NZ_CP028769:c4170026-4169691 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCCGTGCTGATGAAACAACTGGAGTTGAAACTGGCTGACGATGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCAAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA