Detailed information of TA system TA104029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 4166966..4168301
• Replicon: chromosome
• Accession: NZ_CP028769
• Organism: Escherichia coli strain A5

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: AOY56_RS20015
• Protein ID: WP_032251793.1
• Coordinates: 4166966..4167913 (-)
• Length: 316 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: A0A228Z6F8
• Locus tag: AOY56_RS20020
• Protein ID: WP_000152739.1
• Coordinates: 4167906..4168301 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY56_RS20000 (4163099)	4163099..4163641	+	543	WP_001115385.1	hypothetical protein	-
AOY56_RS20005 (4163638)	4163638..4165107	+	1470	WP_001022619.1	serine/threonine-protein kinase	-
AOY56_RS20010 (4165308)	4165308..4166573	-	1266	WP_001218329.1	integrase arm-type DNA-binding domain-containing protein	-
AOY56_RS20015 (4166966)	4166966..4167913	-	948	WP_032251793.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
AOY56_RS20020 (4167906)	4167906..4168301	-	396	WP_000152739.1	DUF6088 family protein	Antitoxin
AOY56_RS20025 (4168371)	4168371..4169204	-	834	WP_086629378.1	DUF4942 domain-containing protein	-
AOY56_RS20030 (4169319)	4169319..4169660	-	342	WP_086629380.1	TA system toxin CbtA family protein	-
AOY56_RS20035 (4169691)	4169691..4170026	-	336	WP_086629383.1	type IV toxin-antitoxin system YeeU family antitoxin	-
AOY56_RS20040 (4170026)	4170026..4170499	-	474	WP_086629385.1	DNA repair protein RadC	-
AOY56_RS20045 (4170529)	4170529..4171347	-	819	WP_001296926.1	DUF932 domain-containing protein	-
AOY56_RS20050 (4171584)	4171584..4172537	-	954	WP_087599172.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimC / fimI / fimA / fimE / fimB	4136680..4186231	49551

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-303)

Antitoxin

• Protein of unknown function DUF6088 (7-108)

Sequences

Toxin

Protein

• Length: 316 a.a.
• Molecular weight: 35604.75 Da
• Isoelectric Point: 4.8847

>T104029 WP_032251793.1 NZ_CP028769:c4167913-4166966 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSQLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYEMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA

Nucleotide

• Length: 948 bp

>T104029 NZ_CP028769:c4167913-4166966 [Escherichia coli]
ATGGATGATTTAACCACTCTTTTTTCTGAAGTGGCTGACGCGCTTGGTATTGAGTCTGTCGCAATTGTGGAAAAAGATCA
CTATATCGTTGAGCTTTTGCAATTGCTTAAACCTCTGGTGTTCGATACTCATCAATTGGTGTTTGCCGGAGGAACCGCTC
TGGCAAAATCAGGTGTCGCACTTAATCGCATGTCGGAAGATGTGGATATCAAACTGGTGCCTAAGGATGCATTTCAGGCA
CTTTCCCGCACTCAGCGAAAAAATACCCGTAAAGAAATTGTTCAGGCAGTTTCCAGTGCAATAGCTTCATCGGGTGTGTT
CAATTTTGATGATGATTACCCAAAAGTGACGCGGGATGAATATCGTTATAATGACATACCTGTTCGTTATCCACAGCGAG
TGGTGCAGGCACCTTGTTTGCGTCCTTTTATCCGGCTGGAATTGATGGAAGCGAAGTTACTGGAGCCTTCTGAATTACGG
GATATTCATTCACTGGTAACAGGGATCACGGGAAAAGCGGCTATTGTTTCAGCGTTTCCATGCGCAACTATCATTAGCAC
ACAGGCAGAAAAGCTGGTGGCGATGTTACGTCGTACTGCGGCAGTTGTGCGAGATGTTGAGCGATTTGATGATGCATCGC
TGGTACGTCATATCTACGATACAAACTGTATTGTTGAGACGAGTCATGTTGATATGAGTCAGCTCGTTGATTTTGTTCAA
CAAACTATTGAGCAGGACATTCAGCGTTACGGCAACCAGTACCCGCAGTTTTGCGCGTCTCCAGTTGATGAGCTAAAAAT
GGGACTGGATGAGTTGGCGAACAATCCTCTCTACGAGATGCGCTATCAGCAGTTTGTTTCGCCAATGGTGTATGGAGAGC
GAAAGATCACATGGGATGAGGCTTACTCCAGGTTTAGGAGCTTAGCGCTAGCCATCCTGAATGCTTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14605.90 Da
• Isoelectric Point: 11.6915

>AT104029 WP_000152739.1 NZ_CP028769:c4168301-4167906 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRDLVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Nucleotide

• Length: 396 bp

>AT104029 NZ_CP028769:c4168301-4167906 [Escherichia coli]
ATGACGATAAAAGCACGCATTCAGTCACGACTGAAACGTTCTAAACGTTACGTATTCACTCGTGACGACTTTAAAGATAT
TGCAGGCTACGATCAGGTTGGTCGGGTATTGCGCGATCTTGTCAGGGAAGGGCAACTGTTGAAGGTTGGTTATGGCGTGT
ATACCAAAGCACGGCGGAACGGTATTACAGGCAAGGTAATGCCAGCGGCTCCCGGAGGCTCTTCAGCGGTGATTGTCGAA
ACGCTGGAACGCCTGAAAGTACGCTATCGTTTTGCTGGCGCAACGCAGGCATACAATAGCGGTACTTCTACGCAGATCCC
AGCTTCACCCGAGATAAAAACCCCCCGGCGTTTTAAACGCGTATTATCTGTAGGGAACAGCAGATTAAATGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A228Z6F8