Detailed information of TA system TA104027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3590308..3591145
• Replicon: chromosome
• Accession: NZ_CP028769
• Organism: Escherichia coli strain A5

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AOY56_RS17375
• Protein ID: WP_000227784.1
• Coordinates: 3590603..3591145 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AOY56_RS17370
• Protein ID: WP_001297137.1
• Coordinates: 3590308..3590619 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY56_RS17345 (3585328)	3585328..3586275	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
AOY56_RS17350 (3586297)	3586297..3588288	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AOY56_RS17355 (3588278)	3588278..3588892	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOY56_RS17360 (3588892)	3588892..3589221	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOY56_RS17365 (3589233)	3589233..3590123	+	891	WP_000971336.1	heme o synthase	-
AOY56_RS17370 (3590308)	3590308..3590619	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AOY56_RS17375 (3590603)	3590603..3591145	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AOY56_RS17380 (3591201)	3591201..3592136	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
AOY56_RS17385 (3592544)	3592544..3593908	+	1365	WP_001000978.1	MFS transporter	-
AOY56_RS17390 (3594036)	3594036..3594527	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
AOY56_RS17395 (3594695)	3594695..3595606	+	912	WP_000705853.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T104027 WP_000227784.1 NZ_CP028769:3590603-3591145 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T104027 NZ_CP028769:3590603-3591145 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT104027 WP_001297137.1 NZ_CP028769:3590308-3590619 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT104027 NZ_CP028769:3590308-3590619 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
TGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3