Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1341768..1342393 Replicon chromosome
Accession NZ_CP028769
Organism Escherichia coli strain A5

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag AOY56_RS06480 Protein ID WP_000911329.1
Coordinates 1341995..1342393 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag AOY56_RS06475 Protein ID WP_000450524.1
Coordinates 1341768..1341995 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY56_RS06450 (1337570) 1337570..1338040 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
AOY56_RS06455 (1338040) 1338040..1338612 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
AOY56_RS06460 (1338758) 1338758..1339636 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
AOY56_RS06465 (1339653) 1339653..1340687 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
AOY56_RS06470 (1340900) 1340900..1341613 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
AOY56_RS06475 (1341768) 1341768..1341995 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
AOY56_RS06480 (1341995) 1341995..1342393 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
AOY56_RS06485 (1342540) 1342540..1343403 + 864 WP_001267498.1 neutral zinc metallopeptidase -
AOY56_RS06490 (1343418) 1343418..1345433 + 2016 WP_087599341.1 tRNA cytosine(34) acetyltransferase TmcA -
AOY56_RS06495 (1345507) 1345507..1346205 + 699 WP_000679823.1 esterase -
AOY56_RS06500 (1346315) 1346315..1346515 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T104012 WP_000911329.1 NZ_CP028769:1341995-1342393 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T104012 NZ_CP028769:1341995-1342393 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT104012 WP_000450524.1 NZ_CP028769:1341768-1341995 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT104012 NZ_CP028769:1341768-1341995 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References