104008

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 315130..315716 Replicon chromosome
Accession NZ_CP028769
Organism Escherichia coli strain A5

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag AOY56_RS01435 Protein ID WP_042014162.1
Coordinates 315130..315360 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AOY56_RS01440 Protein ID WP_000593555.1
Coordinates 315357..315716 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY56_RS01425 (311167) 311167..313902 + 2736 WP_021499416.1 ribosome-associated ATPase/putative transporter RbbA -
AOY56_RS01430 (313902) 313902..315026 + 1125 WP_001216257.1 ABC transporter permease -
AOY56_RS01435 (315130) 315130..315360 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
AOY56_RS01440 (315357) 315357..315716 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AOY56_RS01445 (315836) 315836..316237 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
AOY56_RS01450 (316243) 316243..317049 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
AOY56_RS01455 (317046) 317046..317810 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
AOY56_RS01460 (317810) 317810..318643 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
AOY56_RS01465 (318640) 318640..319584 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T104008 WP_042014162.1 NZ_CP028769:315130-315360 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T104008 NZ_CP028769:315130-315360 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT104008 WP_000593555.1 NZ_CP028769:315357-315716 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT104008 NZ_CP028769:315357-315716 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References