Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4509012..4509509 Replicon chromosome
Accession NZ_CP028768
Organism Escherichia coli strain A6

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY57_RS22290 Protein ID WP_001173343.1
Coordinates 4509012..4509185 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag AOY57_RS22295 Protein ID WP_001312331.1
Coordinates 4509213..4509509 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY57_RS22280 (4505682) 4505682..4507139 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
AOY57_RS22285 (4507376) 4507376..4508893 + 1518 WP_000003805.1 lysine--tRNA ligase -
AOY57_RS22290 (4509012) 4509012..4509185 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY57_RS22295 (4509213) 4509213..4509509 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY57_RS22300 (4509737) 4509737..4510009 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
AOY57_RS22305 (4510021) 4510021..4510251 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
AOY57_RS22310 (4510432) 4510432..4512063 + 1632 WP_001216466.1 sensor histidine kinase -
AOY57_RS22315 (4512060) 4512060..4512779 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T104005 WP_001173343.1 NZ_CP028768:c4509185-4509012 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T104005 NZ_CP028768:c4509185-4509012 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT104005 WP_001312331.1 NZ_CP028768:c4509509-4509213 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT104005 NZ_CP028768:c4509509-4509213 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References