Detailed information of TA system TA104001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2820719..2821200
• Replicon: chromosome
• Accession: NZ_CP028768
• Organism: Escherichia coli strain A6

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: -
• Locus tag: AOY57_RS14075
• Protein ID: WP_201478431.1
• Coordinates: 2820910..2821200 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: -
• Locus tag: AOY57_RS14070
• Protein ID: WP_201478430.1
• Coordinates: 2820719..2820910 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY57_RS14025 (2815764)	2815764..2816459	-	696	WP_201478422.1	ead/Ea22-like family protein	-
AOY57_RS14030 (2816446)	2816446..2816751	-	306	WP_201478423.1	DUF4406 domain-containing protein	-
AOY57_RS14035 (2816748)	2816748..2817029	-	282	WP_201478475.1	DNA-binding protein	-
AOY57_RS14040 (2817080)	2817080..2817808	-	729	WP_201478424.1	DUF1627 domain-containing protein	-
AOY57_RS14045 (2817843)	2817843..2818304	-	462	WP_240726620.1	replication protein P	-
AOY57_RS14050 (2818297)	2818297..2819337	-	1041	WP_201478426.1	DnaT-like ssDNA-binding domain-containing protein	-
AOY57_RS14055 (2819409)	2819409..2819834	-	426	WP_201478427.1	toxin YdaT family protein	-
AOY57_RS14060 (2819818)	2819818..2820093	-	276	WP_201478428.1	helix-turn-helix transcriptional regulator	-
AOY57_RS14065 (2820201)	2820201..2820701	+	501	WP_201478429.1	helix-turn-helix transcriptional regulator	-
AOY57_RS14070 (2820719)	2820719..2820910	+	192	WP_201478430.1	antitoxin	Antitoxin
AOY57_RS14075 (2820910)	2820910..2821200	+	291	WP_201478431.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AOY57_RS14080 (2821474)	2821474..2821629	+	156	WP_000379577.1	DUF1391 family protein	-
AOY57_RS24905 (2821631)	2821631..2821732	+	102	Protein_2774	protein YdfB	-
AOY57_RS14090 (2822688)	2822688..2822876	+	189	WP_100756355.1	cell division inhibition protein DicB	-
AOY57_RS14095 (2822873)	2822873..2823076	+	204	WP_201478433.1	DUF1482 family protein	-
AOY57_RS14100 (2823157)	2823157..2825628	+	2472	WP_201478434.1	exonuclease	-
AOY57_RS14105 (2825693)	2825693..2825941	+	249	WP_000113182.1	excisionase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2784426..2831182	46756

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11132.04 Da
• Isoelectric Point: 9.9854

>T104001 WP_201478431.1 NZ_CP028768:2820910-2821200 [Escherichia coli]
MLPILWLPSARDDLRQIVAYIAKENVPAARRLKIRIETSVLPLTEHPYLYPPSERALGLREIVTHPNYIILYRVTASNIE
IVNVVHSRRQYPNKSC

Nucleotide

• Length: 291 bp

>T104001 NZ_CP028768:2820910-2821200 [Escherichia coli]
ATGCTACCCATTTTATGGCTACCATCTGCTCGCGATGATTTGCGTCAGATCGTAGCCTATATTGCCAAGGAAAATGTTCC
AGCAGCTCGTAGACTAAAAATACGCATTGAAACATCAGTTTTGCCACTTACTGAACACCCTTACCTGTATCCACCAAGCG
AAAGAGCTCTAGGCCTTCGTGAGATAGTGACCCATCCTAACTACATAATACTTTACCGAGTAACAGCATCTAACATCGAG
ATCGTAAATGTAGTTCACTCACGAAGGCAGTATCCAAACAAAAGCTGTTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 6861.68 Da
• Isoelectric Point: 3.9389

>AT104001 WP_201478430.1 NZ_CP028768:2820719-2820910 [Escherichia coli]
MSTALSPIISEFETIEQENSYNEWLRAKVASSLADPRPAIPHDEVMAEMENLIAQIAATSGSV

Nucleotide

• Length: 192 bp

>AT104001 NZ_CP028768:2820719-2820910 [Escherichia coli]
ATGAGCACAGCGCTTTCCCCGATAATTTCAGAATTTGAAACAATCGAACAAGAAAACAGCTATAACGAATGGTTGCGCGC
TAAAGTCGCGTCAAGCCTTGCAGATCCTCGCCCCGCAATACCGCATGACGAGGTGATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGCGACTAGCGGGAGTGTGTAA