Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4527051..4527682 Replicon chromosome
Accession NZ_CP028767
Organism Escherichia coli strain A7

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag AOY58_RS21650 Protein ID WP_001259385.1
Coordinates 4527407..4527682 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AOY58_RS21645 Protein ID WP_000593555.1
Coordinates 4527051..4527410 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY58_RS21620 (4523183) 4523183..4524127 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
AOY58_RS21625 (4524124) 4524124..4524957 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
AOY58_RS21630 (4524957) 4524957..4525721 + 765 WP_001136235.1 nickel import ATP-binding protein NikD -
AOY58_RS21635 (4525718) 4525718..4526524 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
AOY58_RS21640 (4526530) 4526530..4526931 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
AOY58_RS21645 (4527051) 4527051..4527410 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AOY58_RS21650 (4527407) 4527407..4527682 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
AOY58_RS21655 (4527755) 4527755..4528879 - 1125 WP_001314210.1 ABC transporter permease -
AOY58_RS21660 (4528879) 4528879..4531614 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
AOY58_RS21665 (4531611) 4531611..4532678 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T103984 WP_001259385.1 NZ_CP028767:c4527682-4527407 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T103984 NZ_CP028767:c4527682-4527407 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT103984 WP_000593555.1 NZ_CP028767:c4527410-4527051 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT103984 NZ_CP028767:c4527410-4527051 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References