Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3888192..3888846 Replicon chromosome
Accession NZ_CP028767
Organism Escherichia coli strain A7

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag AOY58_RS18450 Protein ID WP_000244772.1
Coordinates 3888192..3888599 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY58_RS18455 Protein ID WP_000354046.1
Coordinates 3888580..3888846 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY58_RS18430 (3884149) 3884149..3885882 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
AOY58_RS18435 (3885888) 3885888..3886598 - 711 WP_000715212.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY58_RS18440 (3886623) 3886623..3887519 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY58_RS18445 (3887631) 3887631..3888152 + 522 WP_001055874.1 flavodoxin FldB -
AOY58_RS18450 (3888192) 3888192..3888599 - 408 WP_000244772.1 protein YgfX Toxin
AOY58_RS18455 (3888580) 3888580..3888846 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY58_RS18460 (3889089) 3889089..3890069 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AOY58_RS18465 (3890146) 3890146..3890805 - 660 WP_000250274.1 hemolysin III family protein -
AOY58_RS18470 (3890969) 3890969..3891280 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
AOY58_RS18475 (3891325) 3891325..3892758 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
AOY58_RS18480 (3892815) 3892815..3893558 - 744 WP_097343884.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T103981 WP_000244772.1 NZ_CP028767:c3888599-3888192 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103981 NZ_CP028767:c3888599-3888192 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103981 WP_000354046.1 NZ_CP028767:c3888846-3888580 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103981 NZ_CP028767:c3888846-3888580 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References