Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3426193..3426818 Replicon chromosome
Accession NZ_CP028767
Organism Escherichia coli strain A7

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag AOY58_RS16200 Protein ID WP_000911329.1
Coordinates 3426193..3426591 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag AOY58_RS16205 Protein ID WP_000450524.1
Coordinates 3426591..3426818 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY58_RS16180 (3422100) 3422100..3422300 + 201 WP_000383836.1 YpfN family protein -
AOY58_RS16185 (3422381) 3422381..3423079 - 699 WP_000679812.1 esterase -
AOY58_RS16190 (3423153) 3423153..3425168 - 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
AOY58_RS16195 (3425183) 3425183..3426046 - 864 WP_001267495.1 neutral zinc metallopeptidase -
AOY58_RS16200 (3426193) 3426193..3426591 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
AOY58_RS16205 (3426591) 3426591..3426818 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
AOY58_RS16210 (3426973) 3426973..3427686 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
AOY58_RS16215 (3427899) 3427899..3428933 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
AOY58_RS16220 (3428950) 3428950..3429828 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
AOY58_RS16225 (3429974) 3429974..3430546 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
AOY58_RS16230 (3430546) 3430546..3431016 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T103979 WP_000911329.1 NZ_CP028767:c3426591-3426193 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T103979 NZ_CP028767:c3426591-3426193 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT103979 WP_000450524.1 NZ_CP028767:c3426818-3426591 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT103979 NZ_CP028767:c3426818-3426591 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References