Detailed information of TA system TA103960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1198309..1199146
• Replicon: chromosome
• Accession: NZ_CP028767
• Organism: Escherichia coli strain A7

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AOY58_RS05545
• Protein ID: WP_000227784.1
• Coordinates: 1198309..1198851 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AOY58_RS05550
• Protein ID: WP_001297137.1
• Coordinates: 1198835..1199146 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY58_RS05525 (1193848)	1193848..1194759	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
AOY58_RS05530 (1194927)	1194927..1195418	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
AOY58_RS05535 (1195546)	1195546..1196910	-	1365	WP_001000960.1	MFS transporter	-
AOY58_RS05540 (1197318)	1197318..1198253	+	936	WP_001368479.1	tetratricopeptide repeat protein	-
AOY58_RS05545 (1198309)	1198309..1198851	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AOY58_RS05550 (1198835)	1198835..1199146	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AOY58_RS05555 (1199331)	1199331..1200221	-	891	WP_000971336.1	heme o synthase	-
AOY58_RS05560 (1200233)	1200233..1200562	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOY58_RS05565 (1200562)	1200562..1201176	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOY58_RS05570 (1201166)	1201166..1203157	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AOY58_RS05575 (1203179)	1203179..1204126	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T103960 WP_000227784.1 NZ_CP028767:c1198851-1198309 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T103960 NZ_CP028767:c1198851-1198309 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT103960 WP_001297137.1 NZ_CP028767:c1199146-1198835 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT103960 NZ_CP028767:c1199146-1198835 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3