Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4666581..4667212 Replicon chromosome
Accession NZ_CP028766
Organism Escherichia coli strain A8

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag AOY61_RS22905 Protein ID WP_001259388.1
Coordinates 4666937..4667212 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AOY61_RS22900 Protein ID WP_000593555.1
Coordinates 4666581..4666940 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY61_RS22870 (4661694) 4661694..4662167 + 474 WP_021554578.1 PTS sugar transporter subunit IIA -
AOY61_RS22875 (4662164) 4662164..4662445 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
AOY61_RS22880 (4662522) 4662522..4663880 + 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -
AOY61_RS22885 (4663873) 4663873..4665381 + 1509 WP_021554579.1 FGGY-family carbohydrate kinase -
AOY61_RS22890 (4665371) 4665371..4665640 + 270 WP_021554580.1 HPr family phosphocarrier protein -
AOY61_RS22895 (4665672) 4665672..4666532 + 861 WP_021554581.1 class II aldolase -
AOY61_RS22900 (4666581) 4666581..4666940 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AOY61_RS22905 (4666937) 4666937..4667212 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
AOY61_RS22910 (4667285) 4667285..4668409 - 1125 WP_096979083.1 ABC transporter permease -
AOY61_RS22915 (4668409) 4668409..4671144 - 2736 WP_096979084.1 ribosome-associated ATPase/putative transporter RbbA -
AOY61_RS22920 (4671141) 4671141..4672208 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4660924..4676064 15140


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T103956 WP_001259388.1 NZ_CP028766:c4667212-4666937 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T103956 NZ_CP028766:c4667212-4666937 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT103956 WP_000593555.1 NZ_CP028766:c4666940-4666581 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT103956 NZ_CP028766:c4666940-4666581 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCTGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References