Detailed information of TA system TA103937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1258572..1259409
• Replicon: chromosome
• Accession: NZ_CP028766
• Organism: Escherichia coli strain A8

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AOY61_RS05930
• Protein ID: WP_000227784.1
• Coordinates: 1258572..1259114 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AOY61_RS05935
• Protein ID: WP_001297137.1
• Coordinates: 1259098..1259409 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY61_RS05910 (1254111)	1254111..1255022	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
AOY61_RS05915 (1255190)	1255190..1255681	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
AOY61_RS05920 (1255809)	1255809..1257173	-	1365	WP_001000955.1	MFS transporter	-
AOY61_RS05925 (1257581)	1257581..1258516	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
AOY61_RS05930 (1258572)	1258572..1259114	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AOY61_RS05935 (1259098)	1259098..1259409	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AOY61_RS05940 (1259594)	1259594..1260484	-	891	WP_000971336.1	heme o synthase	-
AOY61_RS05945 (1260496)	1260496..1260825	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOY61_RS05950 (1260825)	1260825..1261439	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOY61_RS05955 (1261429)	1261429..1263420	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AOY61_RS05960 (1263442)	1263442..1264389	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T103937 WP_000227784.1 NZ_CP028766:c1259114-1258572 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T103937 NZ_CP028766:c1259114-1258572 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT103937 WP_001297137.1 NZ_CP028766:c1259409-1259098 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT103937 NZ_CP028766:c1259409-1259098 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3