Detailed information of TA system TA103930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 3968776..3969371
• Replicon: chromosome
• Accession: NZ_CP028765
• Organism: Escherichia coli strain A9

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: AOY62_RS18785
• Protein ID: WP_000239581.1
• Coordinates: 3968776..3969126 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: AOY62_RS18790
• Protein ID: WP_001223213.1
• Coordinates: 3969120..3969371 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY62_RS18765 (3964150)	3964150..3965172	-	1023	WP_001298067.1	ABC transporter permease	-
AOY62_RS18770 (3965186)	3965186..3966688	-	1503	WP_201477797.1	sugar ABC transporter ATP-binding protein	-
AOY62_RS18775 (3966901)	3966901..3967857	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
AOY62_RS18780 (3968167)	3968167..3968697	+	531	WP_000055075.1	inorganic diphosphatase	-
AOY62_RS18785 (3968776)	3968776..3969126	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
AOY62_RS18790 (3969120)	3969120..3969371	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
AOY62_RS18795 (3969583)	3969583..3969924	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
AOY62_RS18800 (3969927)	3969927..3973706	-	3780	WP_096987568.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T103930 WP_000239581.1 NZ_CP028765:c3969126-3968776 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T103930 NZ_CP028765:c3969126-3968776 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT103930 WP_001223213.1 NZ_CP028765:c3969371-3969120 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT103930 NZ_CP028765:c3969371-3969120 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACCCCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7