Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3239317..3239935 Replicon chromosome
Accession NZ_CP028765
Organism Escherichia coli strain A9

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY62_RS15355 Protein ID WP_001291435.1
Coordinates 3239717..3239935 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY62_RS15350 Protein ID WP_000344800.1
Coordinates 3239317..3239691 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY62_RS15340 (3234406) 3234406..3235599 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOY62_RS15345 (3235622) 3235622..3238771 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
AOY62_RS15350 (3239317) 3239317..3239691 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY62_RS15355 (3239717) 3239717..3239935 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY62_RS15360 (3240107) 3240107..3240658 + 552 WP_000102564.1 maltose O-acetyltransferase -
AOY62_RS15365 (3240774) 3240774..3241244 + 471 WP_000136192.1 YlaC family protein -
AOY62_RS15370 (3241408) 3241408..3242958 + 1551 WP_034173910.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY62_RS15375 (3243000) 3243000..3243353 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY62_RS15385 (3243732) 3243732..3244043 + 312 WP_000409911.1 MGMT family protein -
AOY62_RS15390 (3244074) 3244074..3244646 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103926 WP_001291435.1 NZ_CP028765:3239717-3239935 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103926 NZ_CP028765:3239717-3239935 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103926 WP_000344800.1 NZ_CP028765:3239317-3239691 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103926 NZ_CP028765:3239317-3239691 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References