Detailed information of TA system TA103925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2567992..2568630
• Replicon: chromosome
• Accession: NZ_CP028765
• Organism: Escherichia coli strain A9

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: AOY62_RS12205
• Protein ID: WP_000813795.1
• Coordinates: 2568454..2568630 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AOY62_RS12200
• Protein ID: WP_001270286.1
• Coordinates: 2567992..2568408 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY62_RS12180 (2563144)	2563144..2564085	-	942	WP_001251315.1	ABC transporter permease	-
AOY62_RS12185 (2564086)	2564086..2565099	-	1014	WP_201478290.1	ABC transporter ATP-binding protein	-
AOY62_RS12190 (2565117)	2565117..2566262	-	1146	WP_201478291.1	ABC transporter substrate-binding protein	-
AOY62_RS12195 (2566507)	2566507..2567913	-	1407	WP_201478292.1	PLP-dependent aminotransferase family protein	-
AOY62_RS12200 (2567992)	2567992..2568408	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AOY62_RS12205 (2568454)	2568454..2568630	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AOY62_RS12210 (2568852)	2568852..2569082	+	231	WP_000494244.1	YncJ family protein	-
AOY62_RS12215 (2569174)	2569174..2571135	-	1962	WP_063085534.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AOY62_RS12220 (2571208)	2571208..2571744	-	537	WP_201478293.1	DNA-binding transcriptional regulator SutR	-
AOY62_RS12225 (2571797)	2571797..2573008	+	1212	WP_201478294.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	sodB / fimA / espR1	2274157..2582549	308392

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T103925 WP_000813795.1 NZ_CP028765:c2568630-2568454 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T103925 NZ_CP028765:c2568630-2568454 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT103925 WP_001270286.1 NZ_CP028765:c2568408-2567992 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT103925 NZ_CP028765:c2568408-2567992 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1