Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 932100..932754 Replicon chromosome
Accession NZ_CP028765
Organism Escherichia coli strain A9

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag AOY62_RS04450 Protein ID WP_000244781.1
Coordinates 932347..932754 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY62_RS04445 Protein ID WP_000354046.1
Coordinates 932100..932366 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY62_RS04425 (928188) 928188..929621 - 1434 WP_032281563.1 6-phospho-beta-glucosidase BglA -
AOY62_RS04430 (929666) 929666..929977 + 312 WP_201477859.1 N(4)-acetylcytidine aminohydrolase -
AOY62_RS04435 (930141) 930141..930800 + 660 WP_000250269.1 hemolysin III family protein -
AOY62_RS04440 (930877) 930877..931857 - 981 WP_049589719.1 tRNA-modifying protein YgfZ -
AOY62_RS04445 (932100) 932100..932366 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY62_RS04450 (932347) 932347..932754 + 408 WP_000244781.1 protein YgfX Toxin
AOY62_RS04455 (932794) 932794..933315 - 522 WP_001055874.1 flavodoxin FldB -
AOY62_RS04460 (933427) 933427..934323 + 897 WP_001587400.1 site-specific tyrosine recombinase XerD -
AOY62_RS04465 (934348) 934348..935058 + 711 WP_135425877.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY62_RS04470 (935064) 935064..936797 + 1734 WP_000813188.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T103913 WP_000244781.1 NZ_CP028765:932347-932754 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103913 NZ_CP028765:932347-932754 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103913 WP_000354046.1 NZ_CP028765:932100-932366 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103913 NZ_CP028765:932100-932366 [Escherichia coli]
ATGGATATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References