Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 337410..338041 Replicon chromosome
Accession NZ_CP028765
Organism Escherichia coli strain A9

Toxin (Protein)


Gene name hicA Uniprot ID A0A1M2HXK8
Locus tag AOY62_RS01470 Protein ID WP_072648038.1
Coordinates 337410..337685 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag AOY62_RS01475 Protein ID WP_000593554.1
Coordinates 337682..338041 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY62_RS01455 (332414) 332414..333481 + 1068 WP_000361491.1 HlyD family efflux transporter periplasmic adaptor subunit -
AOY62_RS01460 (333478) 333478..336213 + 2736 WP_201477940.1 ribosome-associated ATPase/putative transporter RbbA -
AOY62_RS01465 (336213) 336213..337337 + 1125 WP_072648039.1 ABC transporter permease -
AOY62_RS01470 (337410) 337410..337685 + 276 WP_072648038.1 type II toxin-antitoxin system HicA family toxin Toxin
AOY62_RS01475 (337682) 337682..338041 + 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AOY62_RS01480 (338091) 338091..338951 - 861 WP_001131788.1 class II aldolase -
AOY62_RS01485 (338983) 338983..339252 - 270 WP_000933060.1 HPr family phosphocarrier protein -
AOY62_RS01490 (339242) 339242..340750 - 1509 WP_001544979.1 FGGY-family carbohydrate kinase -
AOY62_RS01495 (340743) 340743..342101 - 1359 Protein_294 PTS galactitol transporter subunit IIC -
AOY62_RS01500 (342178) 342178..342459 - 282 WP_000084021.1 PTS sugar transporter subunit IIB -
AOY62_RS01505 (342456) 342456..342929 - 474 WP_001306344.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.90 Da        Isoelectric Point: 10.6128

>T103911 WP_072648038.1 NZ_CP028765:337410-337685 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T103911 NZ_CP028765:337410-337685 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT103911 WP_000593554.1 NZ_CP028765:337682-338041 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT103911 NZ_CP028765:337682-338041 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M2HXK8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References