Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4457182..4457679 Replicon chromosome
Accession NZ_CP028764
Organism Escherichia coli strain A10

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY63_RS21335 Protein ID WP_001173343.1
Coordinates 4457182..4457355 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag AOY63_RS21340 Protein ID WP_000398619.1
Coordinates 4457383..4457679 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY63_RS21325 (4453852) 4453852..4455309 + 1458 WP_000856814.1 dipeptide/tripeptide permease DtpC -
AOY63_RS21330 (4455546) 4455546..4457063 + 1518 WP_000003806.1 lysine--tRNA ligase -
AOY63_RS21335 (4457182) 4457182..4457355 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY63_RS21340 (4457383) 4457383..4457679 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY63_RS21345 (4457908) 4457908..4458180 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
AOY63_RS21350 (4458192) 4458192..4458422 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
AOY63_RS21355 (4458603) 4458603..4460234 + 1632 WP_001216471.1 sensor histidine kinase -
AOY63_RS21360 (4460231) 4460231..4460950 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T103909 WP_001173343.1 NZ_CP028764:c4457355-4457182 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T103909 NZ_CP028764:c4457355-4457182 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT103909 WP_000398619.1 NZ_CP028764:c4457679-4457383 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT103909 NZ_CP028764:c4457679-4457383 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGATTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References