Detailed information of TA system TA103908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4357265..4357860
• Replicon: chromosome
• Accession: NZ_CP028764
• Organism: Escherichia coli strain A10

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: AOY63_RS20820
• Protein ID: WP_000239581.1
• Coordinates: 4357265..4357615 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: AOY63_RS20825
• Protein ID: WP_001223213.1
• Coordinates: 4357609..4357860 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY63_RS20800 (4352712)	4352712..4353734	-	1023	WP_001305656.1	ABC transporter permease	-
AOY63_RS20805 (4353748)	4353748..4355250	-	1503	WP_000205837.1	sugar ABC transporter ATP-binding protein	-
AOY63_RS20810 (4355390)	4355390..4356346	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
AOY63_RS20815 (4356656)	4356656..4357186	+	531	WP_000055075.1	inorganic diphosphatase	-
AOY63_RS20820 (4357265)	4357265..4357615	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
AOY63_RS20825 (4357609)	4357609..4357860	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
AOY63_RS20830 (4358072)	4358072..4358413	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
AOY63_RS20835 (4358416)	4358416..4362195	-	3780	WP_201477219.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T103908 WP_000239581.1 NZ_CP028764:c4357615-4357265 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T103908 NZ_CP028764:c4357615-4357265 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT103908 WP_001223213.1 NZ_CP028764:c4357860-4357609 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT103908 NZ_CP028764:c4357860-4357609 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATTTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7