Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3614893..3615511 Replicon chromosome
Accession NZ_CP028764
Organism Escherichia coli strain A10

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY63_RS17315 Protein ID WP_001291435.1
Coordinates 3615293..3615511 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY63_RS17310 Protein ID WP_000344800.1
Coordinates 3614893..3615267 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY63_RS17300 (3609982) 3609982..3611175 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOY63_RS17305 (3611198) 3611198..3614347 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
AOY63_RS17310 (3614893) 3614893..3615267 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY63_RS17315 (3615293) 3615293..3615511 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY63_RS17320 (3615683) 3615683..3616234 + 552 WP_000102564.1 maltose O-acetyltransferase -
AOY63_RS17325 (3616350) 3616350..3616820 + 471 WP_000136192.1 YlaC family protein -
AOY63_RS17330 (3616984) 3616984..3618534 + 1551 WP_001305493.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY63_RS17335 (3618576) 3618576..3618929 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY63_RS17345 (3619308) 3619308..3619619 + 312 WP_000409911.1 MGMT family protein -
AOY63_RS17350 (3619650) 3619650..3620222 - 573 WP_000779833.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103902 WP_001291435.1 NZ_CP028764:3615293-3615511 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103902 NZ_CP028764:3615293-3615511 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103902 WP_000344800.1 NZ_CP028764:3614893-3615267 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103902 NZ_CP028764:3614893-3615267 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References