Detailed information of TA system TA103901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3214750..3215455
• Replicon: chromosome
• Accession: NZ_CP028764
• Organism: Escherichia coli strain A10

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: AOY63_RS15480
• Protein ID: WP_000539521.1
• Coordinates: 3214750..3215136 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AOY63_RS15485
• Protein ID: WP_001280945.1
• Coordinates: 3215126..3215455 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY63_RS15460 (3210754)	3210754..3211380	+	627	WP_001314584.1	glutathione S-transferase GstB	-
AOY63_RS15465 (3211377)	3211377..3212492	-	1116	WP_000555033.1	aldose sugar dehydrogenase YliI	-
AOY63_RS15470 (3212603)	3212603..3212986	-	384	WP_000497137.1	biofilm formation regulator BssR	-
AOY63_RS15475 (3213199)	3213199..3214524	+	1326	WP_139952192.1	30S ribosomal protein S12 methylthiotransferase RimO	-
AOY63_RS15480 (3214750)	3214750..3215136	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AOY63_RS15485 (3215126)	3215126..3215455	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
AOY63_RS15490 (3215525)	3215525..3216853	-	1329	WP_000086873.1	GGDEF domain-containing protein	-
AOY63_RS15495 (3216861)	3216861..3219209	-	2349	WP_001305945.1	EAL domain-containing protein	-
AOY63_RS15500 (3219387)	3219387..3220298	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T103901 WP_000539521.1 NZ_CP028764:3214750-3215136 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T103901 NZ_CP028764:3214750-3215136 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT103901 WP_001280945.1 NZ_CP028764:3215126-3215455 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT103901 NZ_CP028764:3215126-3215455 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6