Detailed information of TA system TA103899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2585955..2586593
• Replicon: chromosome
• Accession: NZ_CP028764
• Organism: Escherichia coli strain A10

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: AOY63_RS12245
• Protein ID: WP_001306887.1
• Coordinates: 2586417..2586593 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AOY63_RS12240
• Protein ID: WP_001270286.1
• Coordinates: 2585955..2586371 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY63_RS12220 (2581107)	2581107..2582048	-	942	WP_001251335.1	ABC transporter permease	-
AOY63_RS12225 (2582049)	2582049..2583062	-	1014	WP_000220407.1	ABC transporter ATP-binding protein	-
AOY63_RS12230 (2583080)	2583080..2584225	-	1146	WP_000047452.1	ABC transporter substrate-binding protein	-
AOY63_RS12235 (2584470)	2584470..2585876	-	1407	WP_000760596.1	PLP-dependent aminotransferase family protein	-
AOY63_RS12240 (2585955)	2585955..2586371	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AOY63_RS12245 (2586417)	2586417..2586593	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AOY63_RS12250 (2586815)	2586815..2587045	+	231	WP_000494241.1	YncJ family protein	-
AOY63_RS12255 (2587137)	2587137..2589098	-	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AOY63_RS12260 (2589171)	2589171..2589707	-	537	WP_000429136.1	DNA-binding transcriptional regulator SutR	-
AOY63_RS12265 (2589760)	2589760..2590971	+	1212	WP_151341113.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T103899 WP_001306887.1 NZ_CP028764:c2586593-2586417 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T103899 NZ_CP028764:c2586593-2586417 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCGTGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT103899 WP_001270286.1 NZ_CP028764:c2586371-2585955 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT103899 NZ_CP028764:c2586371-2585955 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76