Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 971697..972351 Replicon chromosome
Accession NZ_CP028764
Organism Escherichia coli strain A10

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag AOY63_RS04655 Protein ID WP_000244781.1
Coordinates 971944..972351 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY63_RS04650 Protein ID WP_000354046.1
Coordinates 971697..971963 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY63_RS04630 (967785) 967785..969218 - 1434 WP_001305827.1 6-phospho-beta-glucosidase BglA -
AOY63_RS04635 (969263) 969263..969574 + 312 WP_001182966.1 N(4)-acetylcytidine aminohydrolase -
AOY63_RS04640 (969738) 969738..970397 + 660 WP_000250274.1 hemolysin III family protein -
AOY63_RS04645 (970474) 970474..971454 - 981 WP_000886051.1 tRNA-modifying protein YgfZ -
AOY63_RS04650 (971697) 971697..971963 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY63_RS04655 (971944) 971944..972351 + 408 WP_000244781.1 protein YgfX Toxin
AOY63_RS04660 (972391) 972391..972912 - 522 WP_001055888.1 flavodoxin FldB -
AOY63_RS04665 (973024) 973024..973920 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY63_RS04670 (973945) 973945..974655 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY63_RS04675 (974661) 974661..976394 + 1734 WP_000813175.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T103888 WP_000244781.1 NZ_CP028764:971944-972351 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103888 NZ_CP028764:971944-972351 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103888 WP_000354046.1 NZ_CP028764:971697-971963 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103888 NZ_CP028764:971697-971963 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References