Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4231440..4232035 Replicon chromosome
Accession NZ_CP028763
Organism Escherichia coli strain A11

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag AOY64_RS20400 Protein ID WP_000239579.1
Coordinates 4231440..4231790 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag AOY64_RS20405 Protein ID WP_001223208.1
Coordinates 4231784..4232035 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY64_RS20380 (4226886) 4226886..4227908 - 1023 WP_001368084.1 ABC transporter permease -
AOY64_RS20385 (4227922) 4227922..4229424 - 1503 WP_087895020.1 sugar ABC transporter ATP-binding protein -
AOY64_RS20390 (4229564) 4229564..4230520 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
AOY64_RS20395 (4230830) 4230830..4231360 + 531 WP_000055075.1 inorganic diphosphatase -
AOY64_RS20400 (4231440) 4231440..4231790 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
AOY64_RS20405 (4231784) 4231784..4232035 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
AOY64_RS20410 (4232247) 4232247..4232588 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
AOY64_RS20415 (4232591) 4232591..4236370 - 3780 WP_000060896.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T103882 WP_000239579.1 NZ_CP028763:c4231790-4231440 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T103882 NZ_CP028763:c4231790-4231440 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT103882 WP_001223208.1 NZ_CP028763:c4232035-4231784 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT103882 NZ_CP028763:c4232035-4231784 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References