Detailed information of TA system TA103880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3619441..3620278
• Replicon: chromosome
• Accession: NZ_CP028763
• Organism: Escherichia coli strain A11

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AOY64_RS17575
• Protein ID: WP_000227784.1
• Coordinates: 3619736..3620278 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AOY64_RS17570
• Protein ID: WP_001297137.1
• Coordinates: 3619441..3619752 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY64_RS17545 (3614461)	3614461..3615408	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
AOY64_RS17550 (3615430)	3615430..3617421	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AOY64_RS17555 (3617411)	3617411..3618025	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOY64_RS17560 (3618025)	3618025..3618354	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOY64_RS17565 (3618366)	3618366..3619256	+	891	WP_000971336.1	heme o synthase	-
AOY64_RS17570 (3619441)	3619441..3619752	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AOY64_RS17575 (3619736)	3619736..3620278	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AOY64_RS17580 (3620334)	3620334..3621269	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
AOY64_RS17585 (3621677)	3621677..3623041	+	1365	WP_087895014.1	MFS transporter	-
AOY64_RS17590 (3623169)	3623169..3623660	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
AOY64_RS17595 (3623828)	3623828..3624739	+	912	WP_000705848.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T103880 WP_000227784.1 NZ_CP028763:3619736-3620278 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T103880 NZ_CP028763:3619736-3620278 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT103880 WP_001297137.1 NZ_CP028763:3619441-3619752 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT103880 NZ_CP028763:3619441-3619752 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3