Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3585364..3585982 Replicon chromosome
Accession NZ_CP028763
Organism Escherichia coli strain A11

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY64_RS17405 Protein ID WP_001291435.1
Coordinates 3585764..3585982 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY64_RS17400 Protein ID WP_000344800.1
Coordinates 3585364..3585738 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY64_RS17390 (3580453) 3580453..3581646 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOY64_RS17395 (3581669) 3581669..3584818 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AOY64_RS17400 (3585364) 3585364..3585738 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY64_RS17405 (3585764) 3585764..3585982 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY64_RS17410 (3586153) 3586153..3586704 + 552 WP_087895013.1 maltose O-acetyltransferase -
AOY64_RS17415 (3586820) 3586820..3587290 + 471 WP_000136192.1 YlaC family protein -
AOY64_RS17420 (3587454) 3587454..3589004 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY64_RS17425 (3589046) 3589046..3589399 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY64_RS17435 (3589778) 3589778..3590089 + 312 WP_000409911.1 MGMT family protein -
AOY64_RS17440 (3590120) 3590120..3590692 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103879 WP_001291435.1 NZ_CP028763:3585764-3585982 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103879 NZ_CP028763:3585764-3585982 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103879 WP_000344800.1 NZ_CP028763:3585364-3585738 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103879 NZ_CP028763:3585364-3585738 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References