Detailed information of TA system TA103878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2484557..2485195
• Replicon: chromosome
• Accession: NZ_CP028763
• Organism: Escherichia coli strain A11

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: AOY64_RS11940
• Protein ID: WP_000813794.1
• Coordinates: 2485019..2485195 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AOY64_RS11935
• Protein ID: WP_001270286.1
• Coordinates: 2484557..2484973 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY64_RS11915 (2479709)	2479709..2480650	-	942	WP_001251315.1	ABC transporter permease	-
AOY64_RS11920 (2480651)	2480651..2481664	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
AOY64_RS11925 (2481682)	2481682..2482827	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
AOY64_RS11930 (2483072)	2483072..2484478	-	1407	WP_000760615.1	PLP-dependent aminotransferase family protein	-
AOY64_RS11935 (2484557)	2484557..2484973	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AOY64_RS11940 (2485019)	2485019..2485195	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AOY64_RS11945 (2485417)	2485417..2485647	+	231	WP_000494244.1	YncJ family protein	-
AOY64_RS11950 (2485739)	2485739..2487700	-	1962	WP_032255020.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AOY64_RS11955 (2487773)	2487773..2488309	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
AOY64_RS11960 (2488362)	2488362..2489576	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2489616..2490764	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T103878 WP_000813794.1 NZ_CP028763:c2485195-2485019 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T103878 NZ_CP028763:c2485195-2485019 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT103878 WP_001270286.1 NZ_CP028763:c2484973-2484557 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT103878 NZ_CP028763:c2484973-2484557 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB