Detailed information of TA system TA103866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1330823..1331448
• Replicon: chromosome
• Accession: NZ_CP028763
• Organism: Escherichia coli strain A11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AOY64_RS06400
• Protein ID: WP_000911330.1
• Coordinates: 1331050..1331448 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: AOY64_RS06395
• Protein ID: WP_000450524.1
• Coordinates: 1330823..1331050 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY64_RS06370 (1326622)	1326622..1327092	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
AOY64_RS06375 (1327092)	1327092..1327664	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
AOY64_RS06380 (1327810)	1327810..1328688	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AOY64_RS06385 (1328705)	1328705..1329742	+	1038	WP_240728520.1	outer membrane protein assembly factor BamC	-
AOY64_RS06390 (1329955)	1329955..1330668	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
AOY64_RS06395 (1330823)	1330823..1331050	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AOY64_RS06400 (1331050)	1331050..1331448	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
AOY64_RS06405 (1331595)	1331595..1332437	+	843	Protein_1255	neutral zinc metallopeptidase	-
AOY64_RS06410 (1332431)	1332431..1334344	+	1914	WP_240728521.1	tRNA cytosine(34) acetyltransferase TmcA	-
AOY64_RS06415 (1334418)	1334418..1334885	+	468	WP_240728522.1	esterase	-
AOY64_RS06420 (1334837)	1334837..1335283	+	447	Protein_1258	esterase	-
AOY64_RS06425 (1335325)	1335325..1335471	-	147	WP_240728523.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T103866 WP_000911330.1 NZ_CP028763:1331050-1331448 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T103866 NZ_CP028763:1331050-1331448 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT103866 WP_000450524.1 NZ_CP028763:1330823-1331050 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT103866 NZ_CP028763:1330823-1331050 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33