Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4476188..4476685 Replicon chromosome
Accession NZ_CP028762
Organism Escherichia coli strain A12

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY65_RS21650 Protein ID WP_001173343.1
Coordinates 4476188..4476361 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag AOY65_RS21655 Protein ID WP_000398619.1
Coordinates 4476389..4476685 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY65_RS21640 (4472858) 4472858..4474315 + 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -
AOY65_RS21645 (4474552) 4474552..4476069 + 1518 WP_001295074.1 lysine--tRNA ligase -
AOY65_RS21650 (4476188) 4476188..4476361 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY65_RS21655 (4476389) 4476389..4476685 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY65_RS21660 (4476912) 4476912..4477184 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
AOY65_RS21665 (4477196) 4477196..4477426 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
AOY65_RS21670 (4477607) 4477607..4479238 + 1632 WP_001216461.1 sensor histidine kinase -
AOY65_RS21675 (4479235) 4479235..4479954 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - iroB 4434850..4476685 41835


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T103861 WP_001173343.1 NZ_CP028762:c4476361-4476188 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T103861 NZ_CP028762:c4476361-4476188 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT103861 WP_000398619.1 NZ_CP028762:c4476685-4476389 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT103861 NZ_CP028762:c4476685-4476389 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References