Detailed information of TA system TA103860

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4464406..4465240
• Replicon: chromosome
• Accession: NZ_CP028762
• Organism: Escherichia coli strain A12

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: AOY65_RS21605
• Protein ID: WP_000854830.1
• Coordinates: 4464863..4465240 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AOY65_RS21600
• Protein ID: WP_024179143.1
• Coordinates: 4464406..4464774 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY65_RS21565 (4460285)	4460285..4461169	+	885	WP_087906764.1	50S ribosome-binding GTPase	-
AOY65_RS21570 (4461289)	4461289..4461966	+	678	WP_001097370.1	hypothetical protein	-
AOY65_RS21575 (4461972)	4461972..4462202	+	231	Protein_4234	DUF905 family protein	-
AOY65_RS21580 (4462295)	4462295..4463116	+	822	WP_114327813.1	DUF932 domain-containing protein	-
AOY65_RS21585 (4463207)	4463207..4463692	+	486	WP_087906750.1	antirestriction protein	-
AOY65_RS21590 (4463708)	4463708..4463953	+	246	WP_087906751.1	DNA repair protein	-
AOY65_RS21595 (4464022)	4464022..4464243	+	222	WP_000692318.1	DUF987 domain-containing protein	-
AOY65_RS21600 (4464406)	4464406..4464774	+	369	WP_024179143.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AOY65_RS21605 (4464863)	4464863..4465240	+	378	WP_000854830.1	TA system toxin CbtA family protein	Toxin
AOY65_RS21610 (4465237)	4465237..4465434	+	198	Protein_4241	DUF5983 family protein	-
AOY65_RS21615 (4465462)	4465462..4465659	+	198	WP_085948736.1	DUF957 domain-containing protein	-
AOY65_RS21620 (4465744)	4465744..4466586	+	843	WP_001280466.1	DUF4942 domain-containing protein	-
AOY65_RS21625 (4467335)	4467335..4468873	+	1539	WP_001187183.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	iroB	4434850..4476685	41835

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14128.18 Da
• Isoelectric Point: 7.3224

>T103860 WP_000854830.1 NZ_CP028762:4464863-4465240 [Escherichia coli]
MKTLPVLPGQVASSRLSPVEIWQILLSRLLDQHYGLTLDDTPFADERVIEQHIEAGISLCDAVNFLVEKYTLVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEVKR

Nucleotide

• Length: 378 bp

>T103860 NZ_CP028762:4464863-4465240 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGTGGCCAGTTCACGCCTGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
ACGGTTGCTGGACCAGCACTACGGCCTTACGCTGGATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCGGGTATTTCACTGTGCGATGCAGTGAACTTTCTCGTTGAAAAATACACACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCGACTGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCAGGGTAAGCATCCGGAGGTGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13715.57 Da
• Isoelectric Point: 6.7393

>AT103860 WP_024179143.1 NZ_CP028762:4464406-4464774 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT103860 NZ_CP028762:4464406-4464774 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACTAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA