Detailed information of TA system TA103857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3648516..3649353
• Replicon: chromosome
• Accession: NZ_CP028762
• Organism: Escherichia coli strain A12

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AOY65_RS17785
• Protein ID: WP_000227784.1
• Coordinates: 3648811..3649353 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AOY65_RS17780
• Protein ID: WP_001297137.1
• Coordinates: 3648516..3648827 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY65_RS17755 (3643536)	3643536..3644483	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
AOY65_RS17760 (3644505)	3644505..3646496	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AOY65_RS17765 (3646486)	3646486..3647100	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOY65_RS17770 (3647100)	3647100..3647429	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOY65_RS17775 (3647441)	3647441..3648331	+	891	WP_000971336.1	heme o synthase	-
AOY65_RS17780 (3648516)	3648516..3648827	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AOY65_RS17785 (3648811)	3648811..3649353	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AOY65_RS17790 (3649409)	3649409..3650344	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
AOY65_RS17795 (3650752)	3650752..3652116	+	1365	WP_001000978.1	MFS transporter	-
AOY65_RS17800 (3652244)	3652244..3652735	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
AOY65_RS17805 (3652903)	3652903..3653814	+	912	WP_000705853.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T103857 WP_000227784.1 NZ_CP028762:3648811-3649353 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T103857 NZ_CP028762:3648811-3649353 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT103857 WP_001297137.1 NZ_CP028762:3648516-3648827 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT103857 NZ_CP028762:3648516-3648827 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3