Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3614427..3615045 Replicon chromosome
Accession NZ_CP028762
Organism Escherichia coli strain A12

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY65_RS17615 Protein ID WP_001291435.1
Coordinates 3614827..3615045 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY65_RS17610 Protein ID WP_000344800.1
Coordinates 3614427..3614801 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY65_RS17600 (3609516) 3609516..3610709 + 1194 WP_044862765.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOY65_RS17605 (3610732) 3610732..3613881 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AOY65_RS17610 (3614427) 3614427..3614801 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY65_RS17615 (3614827) 3614827..3615045 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY65_RS17620 (3615217) 3615217..3615768 + 552 WP_000102568.1 maltose O-acetyltransferase -
AOY65_RS17625 (3615884) 3615884..3616354 + 471 WP_000136192.1 YlaC family protein -
AOY65_RS17630 (3616518) 3616518..3618068 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY65_RS17635 (3618110) 3618110..3618463 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY65_RS17645 (3618842) 3618842..3619153 + 312 WP_000409911.1 MGMT family protein -
AOY65_RS17650 (3619184) 3619184..3619756 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103856 WP_001291435.1 NZ_CP028762:3614827-3615045 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103856 NZ_CP028762:3614827-3615045 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103856 WP_000344800.1 NZ_CP028762:3614427-3614801 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103856 NZ_CP028762:3614427-3614801 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References