Detailed information of TA system TA103844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1467901..1468526
• Replicon: chromosome
• Accession: NZ_CP028762
• Organism: Escherichia coli strain A12

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AOY65_RS07130
• Protein ID: WP_000911330.1
• Coordinates: 1468128..1468526 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: AOY65_RS07125
• Protein ID: WP_000450524.1
• Coordinates: 1467901..1468128 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY65_RS07100 (1463704)	1463704..1464174	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
AOY65_RS07105 (1464174)	1464174..1464746	-	573	WP_000176191.1	glycine cleavage system transcriptional repressor	-
AOY65_RS07110 (1464892)	1464892..1465770	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AOY65_RS07115 (1465787)	1465787..1466821	+	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
AOY65_RS07120 (1467034)	1467034..1467747	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
AOY65_RS07125 (1467901)	1467901..1468128	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AOY65_RS07130 (1468128)	1468128..1468526	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
AOY65_RS07135 (1468673)	1468673..1469536	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
AOY65_RS07140 (1469551)	1469551..1471566	+	2016	WP_044862802.1	tRNA cytosine(34) acetyltransferase TmcA	-
AOY65_RS07145 (1471640)	1471640..1472338	+	699	WP_000679823.1	esterase	-
AOY65_RS07150 (1472448)	1472448..1472648	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T103844 WP_000911330.1 NZ_CP028762:1468128-1468526 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T103844 NZ_CP028762:1468128-1468526 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT103844 WP_000450524.1 NZ_CP028762:1467901-1468128 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT103844 NZ_CP028762:1467901-1468128 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33