Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1030753..1031407 Replicon chromosome
Accession NZ_CP028762
Organism Escherichia coli strain A12

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag AOY65_RS05045 Protein ID WP_000244772.1
Coordinates 1031000..1031407 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY65_RS05040 Protein ID WP_000354046.1
Coordinates 1030753..1031019 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY65_RS05015 (1026041) 1026041..1026784 + 744 WP_000951964.1 SDR family oxidoreductase -
AOY65_RS05020 (1026841) 1026841..1028274 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
AOY65_RS05025 (1028319) 1028319..1028630 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
AOY65_RS05030 (1028794) 1028794..1029453 + 660 WP_000250274.1 hemolysin III family protein -
AOY65_RS05035 (1029530) 1029530..1030510 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AOY65_RS05040 (1030753) 1030753..1031019 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY65_RS05045 (1031000) 1031000..1031407 + 408 WP_000244772.1 protein YgfX Toxin
AOY65_RS05050 (1031447) 1031447..1031968 - 522 WP_001055874.1 flavodoxin FldB -
AOY65_RS05055 (1032080) 1032080..1032976 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY65_RS05060 (1033001) 1033001..1033711 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY65_RS05065 (1033717) 1033717..1035450 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T103842 WP_000244772.1 NZ_CP028762:1031000-1031407 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103842 NZ_CP028762:1031000-1031407 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103842 WP_000354046.1 NZ_CP028762:1030753-1031019 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103842 NZ_CP028762:1030753-1031019 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References