Detailed information of TA system TA103841

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 836130..836928
• Replicon: chromosome
• Accession: NZ_CP028762
• Organism: Escherichia coli strain A12

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1M0ZYM8
• Locus tag: AOY65_RS04030
• Protein ID: WP_000854904.1
• Coordinates: 836130..836507 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AOY65_RS04035
• Protein ID: WP_032209873.1
• Coordinates: 836554..836928 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY65_RS04000 (831530)	831530..832507	+	978	WP_000633239.1	type II secretion system minor pseudopilin GspK	-
AOY65_RS04005 (832504)	832504..833682	+	1179	WP_000094989.1	type II secretion system protein GspL	-
AOY65_RS04010 (833684)	833684..834220	+	537	WP_000942795.1	GspM family type II secretion system protein YghD	-
AOY65_RS04015 (834501)	834501..835343	-	843	WP_047663674.1	DUF4942 domain-containing protein	-
AOY65_RS04020 (835428)	835428..835625	-	198	WP_000445282.1	DUF957 domain-containing protein	-
AOY65_RS04025 (835645)	835645..836133	-	489	Protein_791	DUF5983 family protein	-
AOY65_RS04030 (836130)	836130..836507	-	378	WP_000854904.1	TA system toxin CbtA family protein	Toxin
AOY65_RS04035 (836554)	836554..836928	-	375	WP_032209873.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AOY65_RS04040 (837091)	837091..837312	-	222	WP_000692311.1	DUF987 domain-containing protein	-
AOY65_RS04045 (837375)	837375..837851	-	477	WP_001186726.1	RadC family protein	-
AOY65_RS04050 (837867)	837867..838352	-	486	WP_000849591.1	antirestriction protein	-
AOY65_RS04055 (838407)	838407..839225	-	819	WP_001234620.1	DUF932 domain-containing protein	-
AOY65_RS04060 (839325)	839325..839558	-	234	WP_001119729.1	DUF905 family protein	-
AOY65_RS04065 (839637)	839637..840092	-	456	WP_000581504.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-118)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14280.26 Da
• Isoelectric Point: 7.2922

>T103841 WP_000854904.1 NZ_CP028762:c836507-836130 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T103841 NZ_CP028762:c836507-836130 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13676.47 Da
• Isoelectric Point: 6.3139

>AT103841 WP_032209873.1 NZ_CP028762:c836928-836554 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADLAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLTCEADTLGSCGYVYLAVYPAQAPATTS

Nucleotide

• Length: 375 bp

>AT103841 NZ_CP028762:c836928-836554 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCTCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGAAATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGATCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGGCACAGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A1M0ZYM8