Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3702671..3703331 Replicon chromosome
Accession NZ_CP028761
Organism Escherichia coli strain A13

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag AOY66_RS17670 Protein ID WP_201475026.1
Coordinates 3702671..3703084 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY66_RS17675 Protein ID WP_000354046.1
Coordinates 3703065..3703331 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY66_RS17650 (3698628) 3698628..3700361 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
AOY66_RS17655 (3700367) 3700367..3701077 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY66_RS17660 (3701102) 3701102..3701998 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY66_RS17665 (3702110) 3702110..3702631 + 522 WP_001055874.1 flavodoxin FldB -
AOY66_RS17670 (3702671) 3702671..3703084 - 414 WP_201475026.1 protein YgfX Toxin
AOY66_RS17675 (3703065) 3703065..3703331 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY66_RS17680 (3703574) 3703574..3704554 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
AOY66_RS17685 (3704631) 3704631..3705290 - 660 WP_000250274.1 hemolysin III family protein -
AOY66_RS17690 (3705454) 3705454..3705765 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
AOY66_RS17695 (3705810) 3705810..3707243 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-133)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 16313.30 Da        Isoelectric Point: 11.7064

>T103835 WP_201475026.1 NZ_CP028761:c3703084-3702671 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRLRWQ
GQEWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 414 bp

>T103835 NZ_CP028761:c3703084-3702671 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTTGCGTTGGCAA
GGGCAGGAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAA
ACGGCAACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAG
AGACGCAAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103835 WP_000354046.1 NZ_CP028761:c3703331-3703065 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103835 NZ_CP028761:c3703331-3703065 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References