Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2746403..2746993 Replicon chromosome
Accession NZ_CP028761
Organism Escherichia coli strain A13

Toxin (Protein)


Gene name pemK Uniprot ID V0VE57
Locus tag AOY66_RS13325 Protein ID WP_000373724.1
Coordinates 2746403..2746735 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag AOY66_RS13330 Protein ID WP_000288812.1
Coordinates 2746736..2746993 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY66_RS13305 (2741866) 2741866..2743128 + 1263 WP_000055690.1 integrase arm-type DNA-binding domain-containing protein -
AOY66_RS13310 (2743267) 2743267..2743725 - 459 WP_000526101.1 IS200/IS605-like element IS200C family transposase -
AOY66_RS13315 (2744677) 2744677..2745555 - 879 WP_000511773.1 integrase domain-containing protein -
AOY66_RS13320 (2745934) 2745934..2746356 + 423 WP_001164104.1 hypothetical protein -
AOY66_RS13325 (2746403) 2746403..2746735 - 333 WP_000373724.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AOY66_RS13330 (2746736) 2746736..2746993 - 258 WP_000288812.1 antitoxin Antitoxin
AOY66_RS13335 (2747091) 2747091..2747282 + 192 WP_077561361.1 hypothetical protein -
AOY66_RS13340 (2747341) 2747341..2747547 - 207 WP_000983730.1 helix-turn-helix transcriptional regulator -
AOY66_RS13345 (2747544) 2747544..2748011 - 468 WP_000201267.1 hypothetical protein -
AOY66_RS13350 (2748244) 2748244..2749380 - 1137 WP_000420971.1 ISAs1-like element ISEc1 family transposase -
AOY66_RS13355 (2749816) 2749816..2751822 + 2007 WP_240775798.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2731653..2776922 45269
- inside IScluster/Tn - - 2743267..2749380 6113


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11724.57 Da        Isoelectric Point: 10.5834

>T103830 WP_000373724.1 NZ_CP028761:c2746735-2746403 [Escherichia coli]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T103830 NZ_CP028761:c2746735-2746403 [Escherichia coli]
ATGGACAGAGGGGAAATCTGGCTGGTTTCACTGGATCCGATTGCAGGTCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCCGTTGTTGTTCCCGTTACCAGCGGCGGAAACTTCGCCCGTG
CAGCCGGTTTTACCGTCTCACTGGACGGTGCGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGAGCCCGGAGCGGTAAGCGACTGGAACGTATACCCGATGCTGTCGTGAATGAAGTGCTGGCCCGACTGGAGGC
CATTCTGAGCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT103830 WP_000288812.1 NZ_CP028761:c2746993-2746736 [Escherichia coli]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT103830 NZ_CP028761:c2746993-2746736 [Escherichia coli]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCCCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGTTGTTGGCGCAGTGCGATCCGCACGCAGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VE57

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References