Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1266490..1267108 Replicon chromosome
Accession NZ_CP028761
Organism Escherichia coli strain A13

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY66_RS05955 Protein ID WP_001291435.1
Coordinates 1266490..1266708 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY66_RS05960 Protein ID WP_000344800.1
Coordinates 1266734..1267108 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY66_RS05920 (1261778) 1261778..1262350 + 573 WP_000779831.1 YbaY family lipoprotein -
AOY66_RS05925 (1262381) 1262381..1262692 - 312 WP_000409908.1 MGMT family protein -
AOY66_RS05935 (1263071) 1263071..1263424 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY66_RS05940 (1263466) 1263466..1265016 - 1551 WP_001441899.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY66_RS05945 (1265180) 1265180..1265650 - 471 WP_000136192.1 YlaC family protein -
AOY66_RS05950 (1265766) 1265766..1266317 - 552 WP_000102539.1 maltose O-acetyltransferase -
AOY66_RS05955 (1266490) 1266490..1266708 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY66_RS05960 (1266734) 1266734..1267108 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY66_RS05965 (1267653) 1267653..1270802 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
AOY66_RS05970 (1270825) 1270825..1272018 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103814 WP_001291435.1 NZ_CP028761:c1266708-1266490 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103814 NZ_CP028761:c1266708-1266490 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103814 WP_000344800.1 NZ_CP028761:c1267108-1266734 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103814 NZ_CP028761:c1267108-1266734 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References