Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 836825..837375 Replicon chromosome
Accession NZ_CP028761
Organism Escherichia coli strain A13

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag AOY66_RS03955 Protein ID WP_001160966.1
Coordinates 837061..837375 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag AOY66_RS03950 Protein ID WP_000125566.1
Coordinates 836825..837058 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY66_RS03925 (831898) 831898..832185 + 288 WP_000203741.1 ferredoxin-like protein FixX -
AOY66_RS03930 (832244) 832244..833575 + 1332 WP_001183207.1 MFS transporter -
AOY66_RS03935 (833683) 833683..834213 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AOY66_RS03940 (834206) 834206..836068 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
AOY66_RS03945 (836260) 836260..836739 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
AOY66_RS03950 (836825) 836825..837058 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AOY66_RS03955 (837061) 837061..837375 + 315 WP_001160966.1 CcdB family protein Toxin
AOY66_RS03960 (837372) 837372..838220 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AOY66_RS03965 (838227) 838227..838604 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AOY66_RS03970 (838607) 838607..839428 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AOY66_RS03975 (839425) 839425..840414 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AOY66_RS03980 (840414) 840414..841700 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T103812 WP_001160966.1 NZ_CP028761:837061-837375 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T103812 NZ_CP028761:837061-837375 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT103812 WP_000125566.1 NZ_CP028761:836825-837058 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT103812 NZ_CP028761:836825-837058 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References