Detailed information of TA system TA103809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 620541..621063
• Replicon: chromosome
• Accession: NZ_CP028761
• Organism: Escherichia coli strain A13

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AOY66_RS02950
• Protein ID: WP_201475157.1
• Coordinates: 620773..621063 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SC69
• Locus tag: AOY66_RS02945
• Protein ID: WP_000212715.1
• Coordinates: 620541..620783 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY66_RS02925 (616252)	616252..617625	+	1374	WP_001219792.1	UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase	-
AOY66_RS02930 (617783)	617783..618334	-	552	WP_000166276.1	ribosome biogenesis factor YjgA	-
AOY66_RS02935 (618428)	618428..619780	+	1353	WP_001162187.1	metalloprotease PmbA	-
AOY66_RS02940 (619963)	619963..620349	+	387	WP_001232246.1	cytochrome b562	-
AOY66_RS02945 (620541)	620541..620783	+	243	WP_000212715.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AOY66_RS02950 (620773)	620773..621063	+	291	WP_201475157.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AOY66_RS02955 (621064)	621064..621528	-	465	WP_001106233.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
AOY66_RS02960 (621686)	621686..623824	-	2139	WP_000187799.1	anaerobic ribonucleoside-triphosphate reductase	-
AOY66_RS02965 (624218)	624218..625873	-	1656	WP_001338059.1	alpha,alpha-phosphotrehalase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-72)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11409.27 Da
• Isoelectric Point: 9.8531

>T103809 WP_201475157.1 NZ_CP028761:620773-621063 [Escherichia coli]
MNYELTFDPRALKEWQKLGVTVREQFKKKLEDALKRPRNPLAKLRDLPDCYKIKLRTQGYRLVYQVNDKELLVLVIAIGK
RENSAVYEDADKRLDE

Nucleotide

• Length: 291 bp

>T103809 NZ_CP028761:620773-621063 [Escherichia coli]
ATGAACTATGAGCTGACGTTTGATCCGCGGGCACTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGCGCTGAAAAGGCCGCGCAATCCATTGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTACGAAGACGCGGATAAACGACTGGATGAGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9125.56 Da
• Isoelectric Point: 5.1369

>AT103809 WP_000212715.1 NZ_CP028761:620541-620783 [Escherichia coli]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Nucleotide

• Length: 243 bp

>AT103809 NZ_CP028761:620541-620783 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9H4B3