Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2321245..2321883 Replicon chromosome
Accession NZ_CP028760
Organism Escherichia coli strain A14

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag AOY67_RS11375 Protein ID WP_000813794.1
Coordinates 2321245..2321421 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AOY67_RS11380 Protein ID WP_001270286.1
Coordinates 2321467..2321883 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY67_RS11355 (2316864) 2316864..2318078 - 1215 WP_001326689.1 BenE family transporter YdcO -
AOY67_RS11360 (2318131) 2318131..2318667 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
AOY67_RS11365 (2318740) 2318740..2320701 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
AOY67_RS11370 (2320793) 2320793..2321023 - 231 WP_000494244.1 YncJ family protein -
AOY67_RS11375 (2321245) 2321245..2321421 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AOY67_RS11380 (2321467) 2321467..2321883 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AOY67_RS11385 (2321962) 2321962..2323368 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
AOY67_RS11390 (2323613) 2323613..2324758 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
AOY67_RS11395 (2324776) 2324776..2325789 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
AOY67_RS11400 (2325790) 2325790..2326731 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T103789 WP_000813794.1 NZ_CP028760:2321245-2321421 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T103789 NZ_CP028760:2321245-2321421 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT103789 WP_001270286.1 NZ_CP028760:2321467-2321883 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT103789 NZ_CP028760:2321467-2321883 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References