Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1251625..1252243 Replicon chromosome
Accession NZ_CP028760
Organism Escherichia coli strain A14

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY67_RS06030 Protein ID WP_001291435.1
Coordinates 1251625..1251843 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY67_RS06035 Protein ID WP_000344800.1
Coordinates 1251869..1252243 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY67_RS05995 (1246914) 1246914..1247486 + 573 WP_000779831.1 YbaY family lipoprotein -
AOY67_RS06000 (1247517) 1247517..1247828 - 312 WP_000409911.1 MGMT family protein -
AOY67_RS06010 (1248207) 1248207..1248560 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY67_RS06015 (1248602) 1248602..1250152 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY67_RS06020 (1250316) 1250316..1250786 - 471 WP_000136192.1 YlaC family protein -
AOY67_RS06025 (1250902) 1250902..1251453 - 552 WP_000102564.1 maltose O-acetyltransferase -
AOY67_RS06030 (1251625) 1251625..1251843 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY67_RS06035 (1251869) 1251869..1252243 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY67_RS06040 (1252789) 1252789..1255938 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AOY67_RS06045 (1255961) 1255961..1257154 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103784 WP_001291435.1 NZ_CP028760:c1251843-1251625 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103784 NZ_CP028760:c1251843-1251625 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103784 WP_000344800.1 NZ_CP028760:c1252243-1251869 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103784 NZ_CP028760:c1252243-1251869 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References