Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3892232..3892886 Replicon chromosome
Accession NZ_CP028759
Organism Escherichia coli strain A15

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag AOY68_RS18525 Protein ID WP_000244781.1
Coordinates 3892232..3892639 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY68_RS18530 Protein ID WP_000354046.1
Coordinates 3892620..3892886 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY68_RS18505 (3888188) 3888188..3889922 - 1735 Protein_3601 single-stranded-DNA-specific exonuclease RecJ -
AOY68_RS18510 (3889928) 3889928..3890638 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY68_RS18515 (3890663) 3890663..3891559 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY68_RS18520 (3891671) 3891671..3892192 + 522 WP_001055874.1 flavodoxin FldB -
AOY68_RS18525 (3892232) 3892232..3892639 - 408 WP_000244781.1 protein YgfX Toxin
AOY68_RS18530 (3892620) 3892620..3892886 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY68_RS18535 (3893129) 3893129..3894109 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
AOY68_RS18540 (3894305) 3894305..3894964 - 660 WP_000250274.1 hemolysin III family protein -
AOY68_RS18545 (3895128) 3895128..3895439 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
AOY68_RS18550 (3895484) 3895484..3896917 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
AOY68_RS18555 (3896974) 3896974..3897717 - 744 WP_000951961.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T103775 WP_000244781.1 NZ_CP028759:c3892639-3892232 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103775 NZ_CP028759:c3892639-3892232 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103775 WP_000354046.1 NZ_CP028759:c3892886-3892620 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103775 NZ_CP028759:c3892886-3892620 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References