Detailed information of TA system TA103773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3447439..3448064
• Replicon: chromosome
• Accession: NZ_CP028759
• Organism: Escherichia coli strain A15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AOY68_RS16425
• Protein ID: WP_000911330.1
• Coordinates: 3447439..3447837 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: AOY68_RS16430
• Protein ID: WP_000450524.1
• Coordinates: 3447837..3448064 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY68_RS16405 (3443317)	3443317..3443517	+	201	WP_000383836.1	YpfN family protein	-
AOY68_RS16410 (3443627)	3443627..3444325	-	699	WP_000679823.1	esterase	-
AOY68_RS16415 (3444399)	3444399..3446414	-	2016	WP_032197281.1	tRNA cytosine(34) acetyltransferase TmcA	-
AOY68_RS16420 (3446429)	3446429..3447292	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
AOY68_RS16425 (3447439)	3447439..3447837	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
AOY68_RS16430 (3447837)	3447837..3448064	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AOY68_RS16435 (3448218)	3448218..3448931	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
AOY68_RS16440 (3449144)	3449144..3450178	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
AOY68_RS16445 (3450195)	3450195..3451073	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AOY68_RS16450 (3451219)	3451219..3451791	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
AOY68_RS16455 (3451791)	3451791..3452261	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3385458..3511173	125715

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T103773 WP_000911330.1 NZ_CP028759:c3447837-3447439 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T103773 NZ_CP028759:c3447837-3447439 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT103773 WP_000450524.1 NZ_CP028759:c3448064-3447837 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT103773 NZ_CP028759:c3448064-3447837 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33