Detailed information of TA system TA103761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1206905..1207742
• Replicon: chromosome
• Accession: NZ_CP028759
• Organism: Escherichia coli strain A15

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: AOY68_RS05565
• Protein ID: WP_201489234.1
• Coordinates: 1206905..1207447 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AOY68_RS05570
• Protein ID: WP_001297137.1
• Coordinates: 1207431..1207742 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY68_RS05545 (1202444)	1202444..1203355	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
AOY68_RS05550 (1203523)	1203523..1204014	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
AOY68_RS05555 (1204142)	1204142..1205506	-	1365	WP_001000978.1	MFS transporter	-
AOY68_RS05560 (1205914)	1205914..1206849	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
AOY68_RS05565 (1206905)	1206905..1207447	-	543	WP_201489234.1	GNAT family N-acetyltransferase	Toxin
AOY68_RS05570 (1207431)	1207431..1207742	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AOY68_RS05575 (1207927)	1207927..1208817	-	891	WP_000971336.1	heme o synthase	-
AOY68_RS05580 (1208829)	1208829..1209158	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOY68_RS05585 (1209158)	1209158..1209772	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOY68_RS05590 (1209762)	1209762..1211753	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AOY68_RS05595 (1211775)	1211775..1212722	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (67-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19751.97 Da
• Isoelectric Point: 7.9572

>T103761 WP_201489234.1 NZ_CP028759:c1207447-1206905 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVCASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T103761 NZ_CP028759:c1207447-1206905 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTATGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT103761 WP_001297137.1 NZ_CP028759:c1207742-1207431 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT103761 NZ_CP028759:c1207742-1207431 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3