Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3954910..3955564 Replicon chromosome
Accession NZ_CP028758
Organism Escherichia coli strain A16

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag AOY69_RS18655 Protein ID WP_000244781.1
Coordinates 3954910..3955317 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY69_RS18660 Protein ID WP_000354046.1
Coordinates 3955298..3955564 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY69_RS18635 (3950865) 3950865..3952598 - 1734 WP_000813202.1 single-stranded-DNA-specific exonuclease RecJ -
AOY69_RS18640 (3952604) 3952604..3953315 - 712 Protein_3635 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY69_RS18645 (3953350) 3953350..3954237 - 888 Protein_3636 site-specific tyrosine recombinase XerD -
AOY69_RS18650 (3954349) 3954349..3954870 + 522 WP_001055874.1 flavodoxin FldB -
AOY69_RS18655 (3954910) 3954910..3955317 - 408 WP_000244781.1 protein YgfX Toxin
AOY69_RS18660 (3955298) 3955298..3955564 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY69_RS18665 (3955807) 3955807..3956787 + 981 WP_000886083.1 tRNA-modifying protein YgfZ -
AOY69_RS18670 (3956864) 3956864..3957523 - 660 WP_000250274.1 hemolysin III family protein -
AOY69_RS18675 (3957687) 3957687..3957998 - 312 WP_044189774.1 N(4)-acetylcytidine aminohydrolase -
AOY69_RS18680 (3958043) 3958043..3959476 + 1434 WP_044191130.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T103755 WP_000244781.1 NZ_CP028758:c3955317-3954910 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103755 NZ_CP028758:c3955317-3954910 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103755 WP_000354046.1 NZ_CP028758:c3955564-3955298 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103755 NZ_CP028758:c3955564-3955298 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References