Detailed information of TA system TA103744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2375571..2376209
• Replicon: chromosome
• Accession: NZ_CP028758
• Organism: Escherichia coli strain A16

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: AOY69_RS11130
• Protein ID: WP_000813794.1
• Coordinates: 2375571..2375747 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AOY69_RS11135
• Protein ID: WP_001270286.1
• Coordinates: 2375793..2376209 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY69_RS11110 (2371190)	2371190..2372404	-	1215	WP_074430998.1	BenE family transporter YdcO	-
AOY69_RS11115 (2372457)	2372457..2372993	+	537	WP_000429152.1	DNA-binding transcriptional regulator SutR	-
AOY69_RS11120 (2373066)	2373066..2375027	+	1962	WP_119458050.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AOY69_RS11125 (2375119)	2375119..2375349	-	231	WP_000494244.1	YncJ family protein	-
AOY69_RS11130 (2375571)	2375571..2375747	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AOY69_RS11135 (2375793)	2375793..2376209	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AOY69_RS11140 (2376288)	2376288..2377694	+	1407	WP_000760666.1	PLP-dependent aminotransferase family protein	-
AOY69_RS11145 (2377939)	2377939..2379084	+	1146	WP_119458051.1	ABC transporter substrate-binding protein	-
AOY69_RS11150 (2379102)	2379102..2380115	+	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
AOY69_RS11155 (2380116)	2380116..2381057	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2370002..2371150	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T103744 WP_000813794.1 NZ_CP028758:2375571-2375747 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T103744 NZ_CP028758:2375571-2375747 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT103744 WP_001270286.1 NZ_CP028758:2375793-2376209 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT103744 NZ_CP028758:2375793-2376209 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• PDB: 6HPB